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DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______

(a) polysaccharide, negatively

(b) polysaccharide, positively

(c) monosaccharide, positively

(d) monosaccharide, negatively

I had been asked this question in an international level competition.

This intriguing question originated from Cultured Cells in Mammals and Restricted Areas in Intact Organisms in chapter Using Other Organisms of Genetic Engineering

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Right answer is (b) polysaccharide, positively

Easiest explanation: There are various methods for the uptake of transformation DNA such as electroporation and DEAE-dextran method. It is a modified polysaccharide and is positively charged. It forms a complex with negatively charged DNA and is taken into cells by endocytosis.

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