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For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment.

(a) calcium chloride

(b) sodium acetate

(c) lithium acetate

(d) magnesium chloride

I had been asked this question in homework.

Query is from Other Systems in section Using Other Organisms of Genetic Engineering

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The correct choice is (c) lithium acetate

The best I can explain: There are mainly three methods which are used for the introduction of exogenous DNA into S.cervisiae. Lithium acetate is added along with polyethylene glycol and heat shock treatment.

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