Login

Remember

Register

CarrierAdda QnA

Q&A
Hot!
Unanswered
Tags
Users
Ask a Question

Home
Genetic Engineering
Protoplast transformation is less time consuming...

Protoplast transformation is less time consuming than lithium acetate for transformation.

← Prev Question Next Question →

+1 vote

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

Protoplast transformation is less time consuming than lithium acetate for transformation.

(a) True

(b) False

The question was asked during an interview.

This is a very interesting question from Other Systems in section Using Other Organisms of Genetic Engineering

genetic engineering
using other organisms
other systems

Facebook Twitter LinkedIn

Please log in or register to answer this question.

1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

Right answer is (b) False

Explanation: Protoplast transformation is a method used for carrying out transformation but is more time consuming than method based on lithium acetate. But protoplast transformation gives a higher efficiency than method based on lithium acetate.

Please log in or register to add a comment.

Related questions

+1 vote

1 answer

The enzyme used for breaking down of sucrose into glucose and fructose is called as _______________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

nmt1+ gene is used for _____ biosynthesis.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

Schizosaccharomyces pombe is yeast and it reproduces by __________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

The promoter from fbp1+ gene is suppressed in the presence of glucose.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

The replacement of one plasmid by another by introduction of the second plasmid into strain containing the first, followed by removal of the first by counterselection is known as __________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

The leu1+ marker encodes which enzyme?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

Promoters can be divided basically into how many types?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

Species which can grow on _______ as a carbon source are called as methylotropic.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

rosy gene is used as a selectable marker for transformation in Drosophila, it produces an enzyme required for the synthesis of ____________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
drosophila

+1 vote

1 answer

For transformation of the chloroplast of higher plants, a vector is used which ______ in the chloroplast.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
organelle transformation

+1 vote

1 answer

Transformation of Chlamydomonas is most efficient in which method?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
algae

+1 vote

1 answer

If gene of interest is inserted into protoplasts but the transformation is not stable, then it is called as __________ expression systems.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
vascular plants

+1 vote

1 answer

DNA can be injected into Caenorhabditis elegans by biolistic transformation. The injected DNA forms arrays of extrochromosomal copies which are stable in nature.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
organelle transformation

+1 vote

1 answer

Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Natural transformation can be relieved by the use of protoplasts, in the presence of osmotic buffer and polyethylene glycol. What are protoplasts?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
bacteria

+1 vote

1 answer

If a host other than E.coli is to be used, what property of DNA to be inserted is disadvantageous?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
bacteria

+1 vote

1 answer

Many mammalian cells contain Thymidine Kinase, the mammalian enzyme uses the analogue _____ than does the viral enzyme.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

T protein is required for replication. It is a product of ____________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

GAL1 is a controllable promoter and is obtained from genes for galactose metabolism. It is known for encoding ______

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
yac and expression systems

+1 vote

1 answer

It is beneficial to use retrovirus for genetic manipulation of cells in the culture.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Autographa californica nuclear polyhedrois virus (AcNPV) is commonly used bacilovirus for infecting cultured cells. The virus is ________ and _______

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells and bacillovirus

Facebook Twitter LinkedIn

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

All categories
Environmental Biotechnology (884)
Environmental Engineering (1.1k)
Enzyme Technology (752)
Farm Machinery (534)
Fermentation Technology (994)
Finite Element Method (481)
Fluid Mechanics (948)
Fluidization Engineering (267)
Food Engineering (984)
Food Packaging Technology (502)
Food Processing Unit Operations (663)
Foundry and Forging (381)
Genetic Engineering (710)
Geotechnical Engineering I (1.2k)
Geotechnical Engineering II (735)
Hazardous Waste Management (880)
Software Architecture (921)
Mass Transfer (499)
Machine Dynamics (772)
Machine Kinematics (912)
Irrigation Engineering (1.2k)
Linear Integrated Circuits (484)
Machine Design (0)
Machine Drawing (0)

Send feedback
About Us
Copyright
Privacy Policy
Terms of Use
Contact Us

Powered by TalkJarvis

...