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Transformation of Chlamydomonas is most efficient in which method?

(a) Lithium acetate method

(b) Electroporation

(c) Protoplast based method

(d) Both electroporation and protoplast based method

The question was posed to me in an international level competition.

Enquiry is from Algae in chapter Using Other Organisms of Genetic Engineering

1 Answer

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Best answer
Correct option is (c) Protoplast based method

To explain: Transformation of Chlamydomonas is most efficient in the protoplast method. In this autolysin is used in order to carry out cell wall degradation.

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