Question

For transformation of the chloroplast of higher plants, a vector is used which ______ in the chloroplast.

(a) doesn’t replicates

(b) replicates

(c) may or may not replicate

(d) replicates under certain specified conditions

This question was addressed to me during an interview for a job.

My question comes from Organelle Transformation topic in portion Using Other Organisms of Genetic Engineering

Answer 1

Correct answer is (a) doesn’t replicates

Easiest explanation: For transformation of the chloroplast of higher plants, a vector is used which doesn’t replicates in the chloroplast. There is a selectable marker which is present and the gene of interest is flanked by chloroplast DNA.