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Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?

(a) Hypoxanthine and thymidine

(b) Thymidine

(c) Aminopterin and thymidine

(d) Hypoxanthine, thymidine and aminopterin (HAT medium)

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The correct option is (d) Hypoxanthine, thymidine and aminopterin (HAT medium)

The explanation: HGPRT deficient cells in the medium containing all three hypoxanthine, thymidine and aminopterin. It is so because aminopterin blocks the endogenous synthesis of purines needed for the synthesis of nucleic acid.

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