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Expression of tTA gene is ________ by doxycycline which is _______ analogue.

(a) activated, tetracycline

(b) inactivated, chloramphenicol

(c) activated, chloramphenicol

(d) inactivated, tetracycline

I got this question in an internship interview.

This interesting question is from Cultured Cells in Mammals and Restricted Areas in Intact Organisms topic in section Using Other Organisms of Genetic Engineering

1 Answer

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Right choice is (d) inactivated, tetracycline

Explanation: Expression of tTA gene is inactivated by doxycyline which is tetracycline analogue. It is very strong and the expression is very less in the presence of doxycycline.

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