Question

Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________

(a) Inverse PCR

(b) Circular PCR

(c) Non-conventional PCR

(d) In-situ PCR

The question was posed to me during an interview.

My question is based upon Modifications in PCR topic in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

Correct choice is (a) Inverse PCR

Easiest explanation: Generally, the amplification is carried out of the region which is flanked by the primers. But in inverse primers amplification is carried out of the region which lies outside the primers.