Question

Which of the following statement is incorrect regarding the cloning of PCR products?

(a) In cloning via restriction enzymes, restriction sites are induced before amplification is carried out

(b) The restriction sites are induced in the primers before annealing

(c) The intermediate molecules are having restriction sites at both ends

(d) The amplified molecules can be cut at both the ends by appropriate enzymes

The question was asked in class test.

Enquiry is from Modifications in PCR in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

Correct option is (c) The intermediate molecules are having restriction sites at both ends

Best explanation: If cloning is done via restriction enzymes, restriction sites are induced before amplification. The restriction sites are induced in the primers before annealing. As the primer binds, the restriction sites are induced at one end of the intermediate molecule. In full length molecules, restriction sites are at both ends. And the amplified molecules can be cut at both the ends by appropriate enzymes.