Question

Which of the statement is incorrect for jumping PCR?

(a) It is used in the case when the DNA fragment is degraded

(b) In this type of PCR, the molecules are not long enough to span between the two primer sites

(c) At the end of first round of synthesis, the extension of the molecule from the primer site to the end of the fragmented molecule takes place

(d) It doesn’t leads to the formation of chimeric product

The question was posed to me during an interview for a job.

Enquiry is from Precautions and Drawbacks in section Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right option is (d) It doesn’t leads to the formation of chimeric product

Explanation: Jumping PCR is used in the case when the molecule is not long enough to span between the two primer sites. At times, the whole amplification doesn’t take place at one go and hence molecule anneals to other fragment having another portion. Thus at times, PCR products longer than the template are designed. But the disadvantage is that it leads to the formation of chimeric products at times.