Question

If amplification of one of the strand is favoured, the modification of PCR is known as __________

(a) single-strand PCR

(b) partial PCR

(c) asymmetric PCR

(d) anchored PCR

This question was posed to me during an internship interview.

I would like to ask this question from Modifications in PCR topic in division Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct choice is (c) asymmetric PCR

For explanation: If the amplification of one of the strand is preferred, it is called asymmetric PCR. This leads to the formation of single stranded products which are having uses such as in sequencing.