Question

If two successive PCR are carried out, it is called as __________

(a) Touch-down PCR

(b) Hot-start PCR

(c) Combined PCR

(d) Nested PCR

I got this question in semester exam.

My question is from Modifications in PCR topic in section Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right option is (d) Nested PCR

To elaborate: Nested PCR is that in which two PCRs are carried out. In the first PCR, it uses a conventional template and the second PCR is carried out using the product of first PCR as a template.