Question

Which of the statement is incorrect for anchored PCR?

(a) Anchored PCR is the modification in which only one piece of the sequence is known and thus one primer

(b) The known sequence is attached to the required region of amplification and then further used as a second priming site

(c) Fragment the sample DNA and ligate it to known sequence

(d) Tails are added enzymatically to the region of known sequence

This question was addressed to me in an interview for job.

I would like to ask this question from Modifications in PCR topic in section Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right answer is (d) Tails are added enzymatically to the region of known sequence

Best explanation: Anchored PCR is based on the fact that only a piece of the sequence is known and thus only one primer. The known sequence is attached to the required region of amplification and is further used as a second priming site. The sample DNA is fragmented and ligated to known sequence. Also, enzymatically tails are added to the sample DNA or to the molecules formed after first round of synthesis. This tail can be further used as a primer.