Question

A sequence is having two ends, 5’ and 3’. Which of the following statements is correct regarding the nature of the ends?

(a) The 5’ end is having hydroxyl group

(b) The 5’ end is having phosphate group

(c) The 3’ end is having phosphate group

(d) Any group can be present at any end

The question was asked during an online exam.

I'm obligated to ask this question of Restriction Endonuclease & Phosphatases in division Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct option is (b) The 5’ end is having phosphate group

To explain: The 5’ end is having a phosphate group. As in a DNA sequence, the 5’ end is characterized by phosphate group and the 3’ end is characterized by a hydroxyl group.