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Type II cuts the sequence in the following way __________

(a) Within the recognition sequence

(b) At 100-1000 nucleotides away from the recognition sequence

(c) At 27-30 nucleotides away from the recognition sequence

(d) It cuts randomly

The question was asked in an online interview.

Asked question is from Restriction Endonuclease & Phosphatases topic in chapter Basics of Genetic Engineering of Genetic Engineering

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Correct option is (a) Within the recognition sequence

For explanation: Recognition sequence is the set of nucleotides which are identified by the enzyme and then it cleaves. In class II, the cleavage is done within the recognition sequence.

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