Question

Why is DNase preferred over restriction endonuclease in some cases?

(a) DNase is preferred over restriction endonuclease in some cases because the latter are not able to recognize some of the restriction sites

(b) DNase is more specific as restriction endonuclease, so the required fragment is obtained

(c) DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments

(d) DNase is abundant in comparison the restriction endonuclease

I got this question in an internship interview.

This interesting question is from Restriction Endonuclease & Phosphatases in division Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct answer is (c) DNase is less specific as compared to restriction endonuclease, hence there are more chances of representation of all the possible fragments

The explanation: As the DNase is less specific than restriction endonuclease, thus it cuts more randomly. It further leads to the representation of more fragments and hence they can be very useful in genomic library construction.