Question

After cleaving the sequence, the nature of the ends created by the type II endonuclease is __________

(a) The ends created are always single stranded

(b) The ends created are always double stranded

(c) Either the ends are single stranded or they are double stranded

(d) One end is single stranded and one end is double stranded

This question was addressed to me in an online quiz.

Enquiry is from Restriction Endonuclease & Phosphatases in chapter Basics of Genetic Engineering of Genetic Engineering

Answer 1

Right answer is (c) Either the ends are single stranded or they are double stranded

To explain: Either the ends are both double stranded or are both single stranded. The double stranded ends are blunt ends whereas the single stranded ends are sticky ends.