Question

How can phosphatase activity terminated prior to a ligation reaction?

(a) By heating

(b) By cooling

(c) By creating vibrations

(d) By alternatively cooling and heating

The question was posed to me at a job interview.

This key question is from Restriction Endonuclease & Phosphatases topic in section Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct choice is (a) By heating

The explanation is: Heating leads to termination of phosphatase activity prior to a ligation reaction because heating leads to the inactivation of the phosphatase activity.