Question

Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers?

(a) DpnI (GA|TC) and Sau3A (|GATC)

(b) BamHI (G|GATCC) and Sau3A (|GATC)

(c) DpnI (GA|TC) and BglII (A|GATCT)

(d) XbaI (T|CTAGA) and BamHI (G|GATCC)

I got this question in examination.

My question is taken from Restriction Endonuclease & Phosphatases topic in division Basics of Genetic Engineering of Genetic Engineering

Answer 1

Correct choice is (b) BamHI (G|GATCC) and Sau3A (|GATC)

The explanation: BamHI on cleavage leaves GATC as the single stranded end and the same end is left by Sau3A as double stranded, though there recognition sequences are different. The other options don’t satisfy the condition.