Question

Which of the following statement is correct regarding partial digestion?

(a) It is defined as the conditions where all the sites in the DNA sequence are not recognized

(b) The number of fragments created by partial digestion are same as that of complete digestion

(c) It is not useful in representation of genomic library

(d) Exactly half of the sites in the DNA are recognized

I got this question during an interview.

My question is from Restriction Endonuclease & Phosphatases in chapter Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct choice is (a) It is defined as the conditions where all the sites in the DNA sequence are not recognized

To elaborate: Partial digestion is the condition where all the sites aren’t recognized and thus the number of fragments created are not same as that of complete digestion. It is very useful in genomic library representation because nearly each and every segment is represented.