Question

Which of the statement is correct for misincorporation?

(a) If direct sequencing has carried this misincorporation is a great problem

(b) The erroneous molecules give strong signals than genuine molecules in case of misincorporation

(c) If the misincorporation in cloned PCR products it is a problem

(d) Even if the error is induced at an early stage it is not incorporated in many sequences

The question was asked in quiz.

I'd like to ask this question from Precautions and Drawbacks topic in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right choice is (c) If the misincorporation in cloned PCR products it is a problem

For explanation I would say: If the misincorporation takes place and direct sequencing is carried out, it is not a major problem. It is so because only small portion of molecules have it and thus the signals are weaker than that of genuine molecules. If the misincorporation is in cloned PCR product, it is of great problem. It is so because if it is included at an early stage, they are incorporated in many sequences.