Question

Choose the correct statement.

(a) Taq polymerase is having high processivity

(b) Processivity is defined in this case as a synthesis of DNA by polymerase

(c) It requires a 5’ end for the elongation to take place

(d) The maximum size of the molecules which can be synthesized is 10kbp

I got this question by my college professor while I was bunking the class.

The query is from The Basic Technique topic in section Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct answer is (b) Processivity is defined in this case as a synthesis of DNA by polymerase

Explanation: Taq polymerase is having low processivity. It means that it falls off from the template before it has synthesized a large piece of DNA. It requires a 3’ OH for carrying out the elongation. The maximum size of the molecules which can be synthesized is 2-4 kbp.