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Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having.

(a) The driver and tracer are mixed in double stranded form

(b) Tracer is in stoichiometric excess in comparison to driver

(c) Tracer and driver are in the same amount

(d) The sequences are allowed to hybridize

I got this question at a job interview.

Enquiry is from Cloning the cDNA and Specialized Libraries in section Libraries Construction of Genetic Engineering

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Right option is (d) The sequences are allowed to hybridize

Explanation: It is about subtractive libraries. The driver and tracer are mixed in single stranded form and driver is in stoichiometric excess in comparison to the driver. It is about tenfold excess. The sequences are mixed and allowed to hybridize.

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