Question

Choose the correct statement if the RNA is non polydenylated.

(a) A collection of chemically synthesized oligonucleotides is used as primers

(b) They are usually tetramer

(c) Unequal quantities of A, G, T and C are used

(d) The primers attach at only specific sequences for first strand synthesis

This question was addressed to me in unit test.

My question is from Cloning the cDNA and Specialized Libraries in chapter Libraries Construction of Genetic Engineering

Answer 1

The correct option is (a) A collection of chemically synthesized oligonucleotides is used as primers

For explanation: As the RNA is non polyadenylated, oilgo-dT primer can’t be used and in place of it a collection of chemically synthesized oligonucleotides is used as primers. They are usually hexamers and are made by equal quantities of A, G, C and T. And thus all hexameric sequences can be synthesized. The primers can attach to the RNA sequences throughout.