Question

RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

(a) Blunt ended ligation

(b) Addition of linkers

(c) Using appropriate restriction enzymes

(d) All the methods can be used equivalently

The question was posed to me in an online quiz.

I'm obligated to ask this question of Cloning the cDNA and Specialized Libraries in chapter Libraries Construction of Genetic Engineering

Answer 1

The correct option is (d) All the methods can be used equivalently

The best explanation: As the products of RNaseH method and homopolymer tailing method generates dsDNA and blunt ended molecules, there are many methods to attach them to vector. Blunt ended ligation, the addition of linkers and restriction enzymes all can be used.