Question

Which of the statement holds true?

(a) The tracer is treated with an enzyme which generates compatible ends

(b) The driver is treated with an enzyme which generates compatible ends

(c) Both driver and tracer are treated with an enzyme to generate compatible ends

(d) Both driver and tracer are treated with an enzyme to generate incompatible ends

I got this question in class test.

The above asked question is from Cloning the cDNA and Specialized Libraries topic in portion Libraries Construction of Genetic Engineering

Answer 1

The correct choice is (a) The tracer is treated with an enzyme which generates compatible ends

To explain I would say: The tracer is treated with an enzyme which generates compatible ends and the driver is treated to generate incompatible ends. It is done so because then driver-driver or driver-tracer hybrids won’t form. Only tracer-tracer hybrids would form.