Question

Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

(a) Polyadenylation should be at 3’ end

(b) Eukaryotic mRNAs are mostly non-polyadenylated

(c) Bacterial mRNAs and organelle mRNAs are polyadenylated

(d) It is carried out by the addition of T residues after synthesis

The question was asked in homework.

The doubt is from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis topic in portion Libraries Construction of Genetic Engineering

Answer 1

The correct choice is (a) Polyadenylation should be at 3’ end

To elaborate: Polyadenylation of RNA species is an important criterion for the production of cDNA species and polyadeylation should be at the 3’ end. Mostly eukaryotic mRNAs are polyadenylated. It is carried out by the addition of A residues after synthesis. Usually, bacterial mRNAs and organelle mRNAs are non-polyadenylated residues.