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The first primer in the case of 3’ RACE is ___________

(a) internal sequence

(b) oligo-dT adaptor molecule

(c) oligo-dA adaptor molecule

(d) adaptor oligo-dT primer

I got this question during an interview.

This interesting question is from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis topic in division Libraries Construction of Genetic Engineering

1 Answer

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Best answer
The correct choice is (b) oligo-dT adaptor molecule

Easiest explanation: The first primer in the case of 3’ RACE is oligo-dT adaptor molecule. The second primer is an internal sequence.

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