Question

Choose the correct statement for RACE.

(a) It stands for Random Amplification of cDNA ends

(b) It is for cloning particular cDNA ends

(c) It is only of one type, which is 5’ RACE

(d) Sequence data is not available in any case

I had been asked this question by my college professor while I was bunking the class.

I want to ask this question from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis topic in chapter Libraries Construction of Genetic Engineering

Answer 1

Right option is (b) It is for cloning particular cDNA ends

The best I can explain: RACE stands for Rapid Amplification of cDNA ends. Sometimes, we wish to clone specific cDNA portion and are having some sequence data.  It is meant for cloning specific cDNA ends and is classified into two types, 5’ RACE and 3’RACE.