Question

Poly A tail from the column is eluted by using high salt concentration.

(a) True

(b) False

I got this question during an internship interview.

This interesting question is from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis in chapter Libraries Construction of Genetic Engineering

Answer 1

Right answer is (b) False

Best explanation: All non-specific mRNA are firstly eluted from the column. The poly A tail is eluted from using low salt concentration, it is so because low salt concentration destabilizes nucleic acids.