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What is the second primer in the case of 5’ RACE?

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What is the second primer in the case of 5’ RACE?

(a) Internal primer

(b) Oligo-dA sequence

(c) Adaptor-oligo-dT primer

(d) Oligo-dT adaptor molecule

The question was asked by my school teacher while I was bunking the class.

My question is from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis topic in portion Libraries Construction of Genetic Engineering

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Best answer
The correct option is (c) Adaptor-oligo-dT primer

Best explanation: The second primer ie the primer used for the synthesis of the second cDNA strand is adaptor-oligo-dT primer. Subsequent PCR is carried out by using internal primer for coding sequence.

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