Question

At times streptavidin is used in place of cellulose. Choose the correct statement for this alternative.

(a) oligo-dT streptavidin conjugate can be extracted by magnetic beads

(b) The magnetic beads are attached with activin

(c) Recovery is not based on the magnetic properties of the beads

(d) The interaction between polyA tail and the oligo-dT is reduced

I had been asked this question during an online interview.

This key question is from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis in division Libraries Construction of Genetic Engineering

Answer 1

Correct option is (a) oligo-dT streptavidin conjugate can be extracted by magnetic beads

The explanation is: If streptavidin is used in the place of cellulose, oligo-dT streptavidin conjugate can be extracted by magnetic beads. These magnetic beads are having biotin attached to them. Recovery is based on the magnetic properties of the beads. The interaction between the polyA tail and the oilgo-dT still remains the basic principle and it is not affected.