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The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

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The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

(a) Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

(b) Along with simple antigen-antibody complex, a substrate is added for easy precipitation

(c) Protein A-Sepharose is added

(d) Protein A-Sepharose binds to IgG antibodies

This question was posed to me in my homework.

My question is based upon Cloning the cDNA and Specialized Libraries in chapter Libraries Construction of Genetic Engineering

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The correct option is (a) Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

Easy explanation: The analysis of polypeptides after addition of mRNA can be carried out by the addition of antibodies. But it is not simply based on antigen-antibody reaction. A substrate for easy precipitation is also added. For this, protein A-Sepharose is added. Protein A-sepharose binds to IgG antibodies. After carrying out centrifugation, it can be pelleted easily.

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