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If physical methods are used, tracer is amplified and is labelled with biotin.

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in Genetic Engineering by (118k points)
If physical methods are used, tracer is amplified and is labelled with biotin.

(a) True

(b) False

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My question is from Cloning the cDNA and Specialized Libraries in division Libraries Construction of Genetic Engineering

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The correct choice is (b) False

Easiest explanation: In the case of physical removal methods, the driver is amplified and is labelled with biotin. After hybridization between tracer and driver, streptavidin is added and it attaches to biotin. Methods such as phenol extraction are used in order to remove streptavidin and sequences attached to it. Thus, tracer sequences which are not attached to driver remain in the aqueous phase only.

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