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in Genetic Engineering by (118k points)
By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

(a) True

(b) False

I have been asked this question during an interview for a job.

My question is taken from Cloning the cDNA and Specialized Libraries topic in chapter Libraries Construction of Genetic Engineering

1 Answer

+1 vote
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Best answer
The correct option is (a) True

The best explanation: Double stranded oligonucleotides are required for the synthesis of second cDNA strand. This double stranded oligonucleotide is synthesized by separately synthesizing two strands and then annealing them.

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