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If the template is double stranded, they need to be separated before annealing of oligonucleotide.

(a) True

(b) False

I had been asked this question by my school teacher while I was bunking the class.

This is a very interesting question from Mutagenesis without PCR topic in section Modifications and Mutagenesis of Genetic Engineering

1 Answer

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Best answer
Right choice is (a) True

Explanation: If the template is double stranded, then the two strands are separated before annealing of the oligonucleotide. This separation is done by heating or alkali denaturation.

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