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Bisulphite ions are used to deaminate ________ residues in _______ DNA.

(a) C, double stranded

(b) C, single stranded

(c) U, double stranded

(d) U, single stranded

The question was asked during an internship interview.

The origin of the question is Mutagenesis without PCR in division Modifications and Mutagenesis of Genetic Engineering

1 Answer

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Correct choice is (b) C, single stranded

To explain I would say: Bisulphite ions are used to deaminate C residues in single stranded DNA. These C residues on deamination give U residues.

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