Question

Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

(a) double stranded ended

(b) circular molecules

(c) single stranded ends

(d) supercoiled molecules

This question was posed to me by my school principal while I was bunking the class.

The above asked question is from Removal and Introduction of Restriction Sites and Generation of Insertions and Deletions in section Modifications and Mutagenesis of Genetic Engineering

Answer 1

Right answer is (c) single stranded ends

The explanation: Small deletions at a restriction site can be generated by cutting and degrading the single stranded ends with an exonuclease.