Question

The extent of deletions can be manipulated by controlling which of them?

(a) Time of incubation only

(b) Amount of nuclease added

(c) They both have a role to play in the extent of the deletion

(d) The amount of deletion which can be carried out for a particular amount of DNA is fixed regardless of the amount of nuclease and incubation time

I got this question in an online quiz.

Query is from Exonucleases in section Basics of Genetic Engineering of Genetic Engineering

Answer 1

Right choice is (c) They both have a role to play in the extent of the deletion

To elaborate: The extent of deletion can be varied by varying the incubation time and the amount of nuclease added. Thus, they both play a role in controlling the extent of deletion.