Question

Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?

(a) RNA dependent DNA polymerase

(b) DNA dependent RNA polymerase

(c) RNA dependent RNA polymerase

(d) DNA dependent DNA polymerase

The question was posed to me during a job interview.

I want to ask this question from Polymerases topic in division Basics of Genetic Engineering of Genetic Engineering

Answer 1

Correct answer is (b) DNA dependent RNA polymerase

To explain: DNA dependent RNA polymerases are very specific for phage promoters and thus they are used for carrying out highly specific transcription. They are often isolated from T3, T7 phages.