Question

Methylase is useful in cloning experiments.

(a) True

(b) False

The question was posed to me by my college professor while I was bunking the class.

My question is from Exonucleases in section Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct answer is (a) True

The explanation: It is useful in cloning experiments because as it methylates DNA, that DNA is protected from that group of restriction enzyme. It means that if we carry out methylation by EcoRI methylase, the DNA won’t be cleaved by the restriction enzyme EcoRI. In cloning experiments, sometimes it is necessary to protect the DNA from cleavage by a particular enzyme.