Question

The ligation reaction is more efficient in which case?

(a) Blunt end ligation

(b) Sticky end ligation

(c) Both have the same efficiency

(d) Depends on the reaction conditions

I had been asked this question in unit test.

This intriguing question comes from Categories of Ligation Reaction in section Basics of Genetic Engineering of Genetic Engineering

Answer 1

The correct option is (b) Sticky end ligation

For explanation: Sticky end ligation is generally more efficient than blunt end ligation. It is so because sticky end ligation is carried out because of complementary base pairing.