Question

Ligation is defined as ______________

(a) Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands

(b) Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands

(c) Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands

(d) Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands

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Asked question is from Categories of Ligation Reaction topic in section Basics of Genetic Engineering of Genetic Engineering

Answer 1

Right option is (c) Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands

Easiest explanation: Ligation is basically the formation of bonds between the ends of two DNA strands. The strands can be single or double. The nature of the bond is phosphodiester, which means that bond is formed between sugar and phosphate. The phosphodiester bond can either be formed between one strand or both the strands.