Question

What is the kinetics of the intramolecular and intermolecular ligation reactions?

(a) Second order kinetics for intramolecular and first order for intermolecular

(b) First order kinetics for intramolecular and second order for intermolecular

(c) Both are first order

(d) Both are second order

This question was posed to me during an interview.

I would like to ask this question from Categories of Ligation Reaction topic in division Basics of Genetic Engineering of Genetic Engineering

Answer 1

Correct choice is (b) First order kinetics for intramolecular and second order for intermolecular

For explanation: As the intramolecular reaction requires only the type of reaction species, the reaction is first order.Whereas, on the other hand intermolecular reaction requires collision between two different species and hence it is second order reaction.