Question

What are the effects of increasing concentration of reaction components?

(a) It increases chances of ligation in both intramolecular and intermolecular reactions

(b) It increases chances of ligation only in intermolecular and no effect on intramolecular

(c) It decreases chances of ligation in intramolecular and increases in that of intermolecular

(d) It decreases chances of ligation in both types of reaction

This question was addressed to me by my college director while I was bunking the class.

Question is taken from Categories of Ligation Reaction topic in division Basics of Genetic Engineering of Genetic Engineering

Answer 1

Right option is (b) It increases chances of ligation only in intermolecular and no effect on intramolecular

To elaborate: As the concentration of reaction components is increased, there are increased chances of ligation in intermolecular reaction because of frequency of collision of two different molecules increases. The intramolecular reaction is unaffected because the probability of meeting the ends of a molecule remains the same.