Login

Remember

Register

CarrierAdda QnA

Q&A
Hot!
Unanswered
Tags
Users
Ask a Question

Home
Genetic Engineering
In a phosphothiorate nucleotide, oxygen atom is...

In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

← Prev Question Next Question →

+1 vote

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

(a) Magnesium

(b) Calcium

(c) Sodium

(d) Sulphur

The question was posed to me in unit test.

The above asked question is from Mutagenesis using PCR and Recovery of Mutated Sequences in division Modifications and Mutagenesis of Genetic Engineering

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

Facebook Twitter LinkedIn

Please log in or register to answer this question.

1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

The correct choice is (d) Sulphur

The explanation is: In a phosphothiorate nucleotide, oxygen atom is replaced by a sulphur atom. Because of this replacement the DNA molecule becomes resistant to attack by nucleases.

Please log in or register to add a comment.

Related questions

+1 vote

1 answer

Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Choose the incorrect statement for the methodology of selection of molecules with mutant sequences.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Choose the correct statement. (a)There are two flanking primers and they are having mutations

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

For the selection of the molecules having mutated sequence, which of the statement is true?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

What is the function of ung gene?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

DpnI cuts ________ strands.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as:

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

Cre can be introduced by crossing it with a strain containing the gene or by infection with virus containing it.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
cre lox excision

+1 vote

1 answer

Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

Down regulation of expression of endogenous genes by transformation with constructs that would generate sense RNA, rather than anti-sense RNA is known as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
post-transcriptional gene silencing

+1 vote

1 answer

In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

The principle of gene disruption is ________ to replace the endogenous chromosomal copy of a gene with __________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
choosing the right mutations
database of mutant lines and gene disruption

+1 vote

1 answer

Double stranded RNA is cleaved by a nuclease called as Dicer and small fragments are generated known as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
post-transcriptional gene silencing

+1 vote

1 answer

Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

Introduction into host organism can also be done by using a DNA construct, which when transcribed, generates an RNA which is _________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
post-transcriptional gene silencing

+1 vote

1 answer

RNA molecule with catalytic activity is termed as________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
cre lox excision

+1 vote

1 answer

Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis without pcr

+1 vote

1 answer

The process of RNA inactivation by siRNAs is termed as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
post-transcriptional gene silencing

+1 vote

1 answer

Excision of DNA flanked by loxP sequences is also known as _________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
cre lox excision

+1 vote

1 answer

At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

Facebook Twitter LinkedIn

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

All categories
Environmental Biotechnology (884)
Environmental Engineering (1.1k)
Enzyme Technology (752)
Farm Machinery (534)
Fermentation Technology (994)
Finite Element Method (480)
Fluid Mechanics (948)
Fluidization Engineering (267)
Food Engineering (984)
Food Packaging Technology (502)
Food Processing Unit Operations (663)
Foundry and Forging (381)
Genetic Engineering (710)
Geotechnical Engineering I (1.2k)
Geotechnical Engineering II (735)
Hazardous Waste Management (880)
Software Architecture (921)
Mass Transfer (499)
Machine Dynamics (772)
Machine Kinematics (912)
Irrigation Engineering (1.2k)
Linear Integrated Circuits (404)
Machine Design (0)
Machine Drawing (0)

Send feedback
About Us
Copyright
Privacy Policy
Terms of Use
Contact Us

Powered by TalkJarvis

...