Ask a Question

The loop region is single stranded. It can be cleaved by using which enzyme?

← Prev Question Next Question →
+1 vote
in Genetic Engineering by (118k points)
The loop region is single stranded. It can be cleaved by using which enzyme?

(a) Exonuclease

(b) S1 nuclease

(c) RNaseH

(d) DNase

I got this question in an interview for internship.

I would like to ask this question from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis in chapter Libraries Construction of Genetic Engineering

1 Answer

+1 vote
by (693k points)
selected by
 
Best answer
Right option is (b) S1 nuclease

Easy explanation: The loop region in the hairpin structure is single stranded in nature. The single stranded portion can be cleaved by S1 nuclease.

Related questions

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

Powered by TalkJarvis
...