Question

Amplification of specific region can be done by using primers for specific regions. If the PCR product is ______ and is in sufficient quantity, then sequence can be determined ________

(a) non-specific, directly

(b) non-specific, indirectly or directly

(c) specific, directly

(d) specific, indirectly

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Origin of the question is Applications of Genetic Engineering in division Genetic Engineering of Genetic Engineering

Answer 1

The correct answer is (c) specific, directly

Easiest explanation: Amplification of specific region can be done by using primers for specific regions. If the PCR product is specific, it means that only a single band is obtained in gel electrophoresis and is insufficient quantity then the sequence can be determined directly.