Login

Remember

Register

CarrierAdda QnA

Q&A
Hot!
Unanswered
Tags
Users
Ask a Question

Home
Genetic Engineering
For getting a large amount of proteins to...

For getting a large amount of proteins to crystallize, which of the following should be used as an expression system?

← Prev Question Next Question →

+1 vote

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

For getting a large amount of proteins to crystallize, which of the following should be used as an expression system?

(a) Bacterial system

(b) Yeast systems

(c) Eukaryotic systems

(d) Both eukaryotic and bacterial systems can be used

The question was posed to me during an online interview.

The query is from Applications of Genetic Engineering topic in portion Genetic Engineering of Genetic Engineering

genetic engineering
applications of genetic engineering

Facebook Twitter LinkedIn

Please log in or register to answer this question.

1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

Correct answer is (d) Both eukaryotic and bacterial systems can be used

Best explanation: The system to be used for getting large amounts of proteins to crystallize can be either bacterial or eukaryotic. It depends on the source of the gene and whether post-translational modification is necessary or not.

Please log in or register to add a comment.

Related questions

+1 vote

1 answer

If a putative protein sequence is cloned in an expression vector and the expressed protein is not showing protease activity, then which of the following is not correct?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
applications of genetic engineering

+1 vote

1 answer

If a full proteomic analysis of growth medium is carried out and is combined with ____________ genome sequence, genes for other _____________ proteins are also obtained.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
applications of genetic engineering

+1 vote

1 answer

Amplification of specific region can be done by using primers for specific regions. If the PCR product is ______ and is in sufficient quantity, then sequence can be determined ________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
applications of genetic engineering

+1 vote

1 answer

Which of the following is not suitable if the PCR product is non-specific?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
applications of genetic engineering

+1 vote

1 answer

How many approaches are there in order to clone the complete genome?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
applications of genetic engineering

+1 vote

1 answer

If a mutation perturbs the structure, then stability and folding are not affected.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
applications of genetic engineering

+1 vote

1 answer

The disadvantage in the approach based on using PCR is that there is no permanent record until some of the bacterial cells are preserved.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
applications of genetic engineering

+1 vote

1 answer

The RNA level ___________ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ____________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
applications of genetic engineering

+1 vote

1 answer

For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

For the production of unlabelled and huge amount of proteins, which of the following is true?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
synthesis of proteins and translation in vitro

+1 vote

1 answer

For co-expression of proteins, it is possible to have several independent sets of promoters and other signals in a single vector.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
co-expression of proteins and optimization

+1 vote

1 answer

Which of the following are advantageous to be used for expression of eukaryotic cells?

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
yac and expression systems

+1 vote

1 answer

Promoters such as GAL1 can be used to direct synthesis of either unmodified or fusion proteins.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
yac and expression systems

+1 vote

1 answer

A considerable amount of modification can take place in the ______ region of RNA in _______ before translation. It affects the yield of proteins.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
co-expression of proteins and optimization

+1 vote

1 answer

If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
expression of coding section in-vitro

+1 vote

1 answer

The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells in mammals and restricted areas in intact organisms

+1 vote

1 answer

Expression of proteins using nuclear polyhedrosis viruses is advantageous because it gives _______ protein yields and post-translational modifications are _________

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
cultured cells and bacillovirus

+1 vote

1 answer

When lacUV5 control system is used, addition of IPTG _______ the expression of _________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
promoters

+1 vote

1 answer

If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

For mutagenesis without PCR, which of the following can be used as a template?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis without pcr

+1 vote

1 answer

If the amount of agarose added is more, the molecular under analysis should have the following characteristic:

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
basics of genetic engineering
gel electrophoresis
oligonucleotide & microarrays

+1 vote

1 answer

Lon protease is used for degradation of foreign proteins in E.coli cells. It is active against which type of proteins?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
co-expression of proteins and optimization

+1 vote

1 answer

In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Adsorption onto a solid phase support followed by elution is used as an alternative for separation of which component?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
basics of genetic engineering
purification of plasmid dna

Facebook Twitter LinkedIn

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

All categories
Environmental Biotechnology (884)
Environmental Engineering (1.1k)
Enzyme Technology (752)
Farm Machinery (534)
Fermentation Technology (994)
Finite Element Method (481)
Fluid Mechanics (948)
Fluidization Engineering (267)
Food Engineering (984)
Food Packaging Technology (502)
Food Processing Unit Operations (663)
Foundry and Forging (381)
Genetic Engineering (710)
Geotechnical Engineering I (1.2k)
Geotechnical Engineering II (735)
Hazardous Waste Management (880)
Software Architecture (921)
Mass Transfer (499)
Machine Dynamics (772)
Machine Kinematics (912)
Irrigation Engineering (1.2k)
Linear Integrated Circuits (404)
Machine Design (0)
Machine Drawing (0)

Send feedback
About Us
Copyright
Privacy Policy
Terms of Use
Contact Us

Powered by TalkJarvis

...