Question

When lacUV5 control system is used, addition of IPTG _______ the expression of _________

(a) activates, T7 RNA polymerase

(b) inactivates, T7 RNA polymerase

(c) activates, T7 DNA polymerase

(d) inactivates,  T7 DNA polymerase

I have been asked this question in class test.

I would like to ask this question from Promoters topic in portion Use of Cloned DNA of Genetic Engineering

Answer 1

Correct option is (a) activates, T7 RNA polymerase

For explanation: When a lacUV5 control system is used, the addition of IPTG activates the expression of T7 RNA polymerase. Thus transcription of sequences under the control of T7 promoter is controlled.